Abstract:
Acmella oleracea commonly referred to as the toothache effect, has been shown to provide therapeutic benefits by researches. The purpose of the study was to perform phytochemical screening and specific compounds present in the ethanolic extract of A. oleracea (EAO). This study was also designed for performing in vitro antioxidant, thrombolytic and in vivo analgesic and antidiarrheal activity of EAO. Phytochemical screening was done by following the standard method. Compound profiling was executed by GC-MS analysis. The antioxidant potential of EAO was performed by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay with determination of secondary metabolites (TPC, TFC, TTC). Thrombolytic activity was performed using an in vitro streptokinase assay. Castor oil-induced antidiarrheal assay in mice was performed for evaluation of antidiarrheal potential of EAO and acetic acid-induced writhing assay in mice was done for assessment of its analgesic activity. Numerous secondary metabolites, including alkaloids, flavonoids, phenols, tannins, saponins, and terpenoids, were found in EAO. GC-MS profiling revealed the presence of a total 84 compounds. TPC, TFC and TTC were 159.33 ± 6.89 mg GAE/g, 139.42 ± 4.50 mg QE/g and 124.11 ± 5.46 mg GAE/g, respectively. EAO showed comparative DPPH free radical scavenging potential with standard ascorbic acid. The calculated IC50 value was 62.01 μg/mL for EAO and 32.33 μg/mL for ascorbic acid. The thrombolytic potential of standard streptokinase and EAO were found 69.00 % and 43.87 %. Significant .
