Phytochemical Screening and Biological Tests of Methanol Extract of Ruellia simplex

Abstract

The phytochemical composition and possible biological activities of Ruellia simplex, a medicinal herb that has conventional uses, were studied. Plant material was extracted using methanol, and the resultant extract was thoroughly screened for the existence of several secondary metabolites using phytochemical analysis. Alkaloids, flavonoids, Saponin, Glycoside, and tannins were found in the results of the research, indicating the plant’s varied chemical composition. The biochemical properties of the Ruellia simplex methanol extract were then assessed using a battery of in vitro experiments and in-vivo anti-diarrheal, in-vitro thrombolytic activity, in vitro antioxidant & in vivo anthelminthic activity. Ruellia Simplex methanol extract showed adequate thrombolytic effectiveness (69%), according to this investigation. Standard ascorbic acid’s DPPH inhibitory effect displays the IC50 (9.072 μg/ml) as the positive control. The Methanol extract of Ruellia Simplex has a notable DPPH inhibitory effect, with an IC50 of 20.487 μg/ml when compared to standard. The findings of this research emphasize Ruellia simplex’s potential uses in medicine and healthcare by providing significant details on the phytochemical makeup of the plant and its biological activities. The results highlight the significance of looking for novel therapeutic compounds in natural sources and may open the door for future studies.