"Synergistic Effects of Colocasia esculenta, Spinacia oleracea, and Yogurt as a Synbiotic Combination on Hemoglobin Levels, Body Weight Reduction, and Clotting Time: An Experimental Study in a Mice Model

Abstract

Iron deficiency and metabolic imbalances remain significant global health concerns, often exacerbated by limited iron bioavailability in plant-based diets due to inhibitors like phytates and oxalates. This study evaluates the efficacy of a synbiotic combination of Colocasia esculenta (Taro), Spinacia oleracea (Spinach), and yogurt in improving hemoglobin levels, regulating body weight, and enhancing clotting time in a non-anemic mice model. The primary aim was to assess the impact of the synbiotic combination on hemoglobin levels, body weight, and clotting time, and to compare its performance against standalone plant-based interventions and synthetic supplementation. Six groups of mice (Control, Colocasia, Spinach, Probiotic [yogurt], Treatment [synbiotic combination], and Standard [synthetic iron supplement]) were monitored for 14 days. Key parameters—hemoglobin levels, body weight changes, and clotting time—were measured. Statistical analysis, including ANOVA and post-hoc tests, was conducted to determine group differences. The Treatment Group achieved the highest hemoglobin levels (16.75 g/dL, F = 11.18, p < 0.0001), significant weight reduction (-5.50 g, F = 28.76, p < 0.0001), and the shortest clotting time (32.75 seconds, F = 11.18, p < 0.0001). The synbiotic combination demonstrated superior efficacy compared to standalone interventions and synthetic supplementation, emphasizing the role of probiotics in enhancing non-heme iron absorption, improving metabolic efficiency, and supporting systemic health. This study underscores the potential of a synbiotic combination of Colocasia esculenta, Spinacia oleracea, and yogurt to improve hematological and metabolic parameters. The findings suggest that such dietary strategies offer sustainable and effective alternatives to synthetic supplementation. Future research should focus on isolating bioactive compounds, understanding their mechanisms of action, and validating these results through human clinical trials.