Abstract:
This research work may help to board field to improve the medicinal uses of the
Tabernaemontana divaricata leaves. This plant belonging to the family Apocynaceae. The
methanolic extract of Tabernaemontana divaricata leaves chosen for the evaluation of
Phytochemical screening, In-vitro Antioxidant potential. In-vivo Analgesic activity,
Antidiarrheal activity & anthelmintic activity & Ex-vivo Thrombolytic activity. The
Phytochemical studies were done as qualitative analysis. That means this only indicates the
presence or absence of the chemical compounds not the quantity of the chemical compounds.
The phytochemical studies were performed based on the colour change, precipitation & ring
formation etc. The antioxidant activity was determined by using DPPH (1,1-diphenyl-2-
picrylhydrazine) Radical Scavenging Assay for extract (25.93µg/ml) compared with the
standard Ascorbic acid (18.40µg/ml). And also performed reducing sugar capacity, total
flavonoid content (49.59mg/gm) & total phenolic content (54.89mg/gm) in the extract. Exvivo thrombolytic activity was determined by clot disruption method & showed % clot lysis
of methanolic extract showed (48.33%) where standard streptokinase showed (75.44%).
Analgesic activities were performed by in-vivo test and provided a good result. Standard
showed (72.22%) protection of writhing where the extract showed (58.33%) protection of
writhing. Anthelmintic effect of this extract was good and this plant extract have also some
antidiarrheal activities. In my studies, I was found some chemical compounds such as
Phenols, Glycosides, Flavonoids, reducing sugar, Terpinoids & Alkaloids in the methanolic
extract of Tabernaemontana divaricata leaves. So my research work tries to show the
bioactivity of the methanolic extract of Tabernaemontana divaricata leaves.
