Phytochemical screening & Pharmacological Evaluation of the Methanol Extract of Tabernaemontana divaricata leaves.

Abstract

This research work may help to board field to improve the medicinal uses of the Tabernaemontana divaricata leaves. This plant belonging to the family Apocynaceae. The methanolic extract of Tabernaemontana divaricata leaves chosen for the evaluation of Phytochemical screening, In-vitro Antioxidant potential. In-vivo Analgesic activity, Antidiarrheal activity & anthelmintic activity & Ex-vivo Thrombolytic activity. The Phytochemical studies were done as qualitative analysis. That means this only indicates the presence or absence of the chemical compounds not the quantity of the chemical compounds. The phytochemical studies were performed based on the colour change, precipitation & ring formation etc. The antioxidant activity was determined by using DPPH (1,1-diphenyl-2- picrylhydrazine) Radical Scavenging Assay for extract (25.93µg/ml) compared with the standard Ascorbic acid (18.40µg/ml). And also performed reducing sugar capacity, total flavonoid content (49.59mg/gm) & total phenolic content (54.89mg/gm) in the extract. Exvivo thrombolytic activity was determined by clot disruption method & showed % clot lysis of methanolic extract showed (48.33%) where standard streptokinase showed (75.44%). Analgesic activities were performed by in-vivo test and provided a good result. Standard showed (72.22%) protection of writhing where the extract showed (58.33%) protection of writhing. Anthelmintic effect of this extract was good and this plant extract have also some antidiarrheal activities. In my studies, I was found some chemical compounds such as Phenols, Glycosides, Flavonoids, reducing sugar, Terpinoids & Alkaloids in the methanolic extract of Tabernaemontana divaricata leaves. So my research work tries to show the bioactivity of the methanolic extract of Tabernaemontana divaricata leaves.