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Facile and Selective Determination of Dipeptides Using 3-Methylcatechol as a Novel Fluorogenic Reagent

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dc.contributor.author Yasmin, Hasina
dc.contributor.author Rahman, Mohammed Shafikur
dc.contributor.author Shibata, Takayuki
dc.date.accessioned 2019-05-16T05:06:49Z
dc.date.available 2019-05-16T05:06:49Z
dc.date.issued 2018-03-31
dc.identifier.issn 1573-3149
dc.identifier.uri http://hdl.handle.net/123456789/61
dc.description.abstract Selective determination of peptides in mixtures or biological samples requires specific techniques for analysis. Herein, we report 3-methylcatechol (3-MC) as a novel fluorogenic reagent for the selective determination of dipeptides by a simple fluorescence (FL) derivatization reaction. After extensive screening of 31 different catechol analogues, 3-MC was found to generate FL with peptides. The reaction was performed at 100 °C for 10 min in the presence of borate buffer (pH 7) and sodium periodate. The resulting FL intensities were measured by spectrofluorometer at excitation wavelengths of 380 nm and emission wavelengths of 500 nm. Different reaction conditions such as concentration of sodium periodate, reaction time and pH of the borate buffer were studied to determine the optimum reaction conditions. Linearity was obtained between FL intensity and peptide concentrations from 10 to 160 µM with a lower detection limit of 10 µM (S/N = 3). Dipeptides containing Ala, Phe, Leu and Val at the N-termini generated significant FL in comparison to the reagent blank (**p < 0.005, ***p < 0.0005). The reaction is simple, rapid, selective and sensitive which can be applied for the determination of the dipeptides as biomarkers or to determine enzyme activity. en_US
dc.language.iso en_US en_US
dc.publisher Springer Nature en_US
dc.subject Peptides en_US
dc.subject 3-Methylcatechol en_US
dc.subject Fluorescence en_US
dc.subject Selectivity en_US
dc.title Facile and Selective Determination of Dipeptides Using 3-Methylcatechol as a Novel Fluorogenic Reagent en_US
dc.type Article en_US

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