Abstract:
For a six months period a total of 25
Klebisiella sp. samples collected from different
diagnostic centers were tested for their antimicrobial
susceptibility pattern against 16 antimicrobials. All
isolates under study showed resistance against
cephalexin, cephradine, ciporfloxacin, cloxacillin,
erythromycin, oxacillin, rifampicin and tetracycline.
Most of the isolates were resistant to amoxicillin
(92%), vancomycin (96%), neomycin (84%) and
chloramphenicol (76%). Least number of isolates
showed resistance to tetracycline (36%), ceftriaxone
(40%) and gentamycin (56%), respectively. Out of the
25 isolates, 7 multidrug resistant isolates were
selected and they were grown in varying
concentrations of Sodium Dodecyl Sulfate (SDS) in
Luria broth and their plasmid profile was analysed.
Distinct bands of plasmid were observed when the
multidrug resistant strains of Klebsiella sp. were
grown in 5% SDS and acridine orange (10μg/ml) in
Luria broth. On being treated with 5% SDS and 5%
SDS along with acridine orange, one isolate (isolate
no. 3) developed sensitivity against ceftriaxone,
ciprofloxacin, co-trimoxazole, gentamycin and
neomycin.
