MOLECULAR SCREENING OF TRICHODERMA ISOLATES

Bajwa, Amna Ali, Rukhsana

dc.contributor.author	Bajwa, Amna Ali, Rukhsana
dc.date.accessioned	2013-03-20T09:40:25Z
dc.date.accessioned	2019-05-29T05:10:01Z
dc.date.available	2013-03-20T09:40:25Z
dc.date.available	2019-05-29T05:10:01Z
dc.date.issued	2013-01
dc.identifier.uri	http://hdl.handle.net/20.500.11948/871
dc.description.abstract	The molecular study is based on genomic DNA therefore DNA must be extracted in a pure and uncontaminated form. In this study, genomic DNA of twenty four isolates of Trichoderma species (T. harzianum, T. hamatum, T. koningii and T. pseudokoningii) indicated band of ~15Kb on 0.8% agarose gel and quality was determined by obtaining absorbance ratio (260/280) in the range of 1.7-1.9. Restriction fragment length polymorphism (RFLP) analyses were performed by using two restriction endonulease enzymes i.e., BamHI and HindIII. The BamHI represented results in the range of 500bp-750bp. 18S rRNA gene targeting was further carried out through optimization in ribotyping analysis.	en_US
dc.language.iso	en	en_US
dc.publisher	Daffodil International University	en_US
dc.subject	Trichoderma harzianum, T. hamatum, T. koningii, T. pseudokoningii RFLP, restriction endonuleases, 18S rRNA.	en_US
dc.title	MOLECULAR SCREENING OF TRICHODERMA ISOLATES	en_US
dc.type	Article	en_US